Journal: Scientific Reports

Article Title: Dietary polyamines promote intestinal adaptation in an experimental model of short bowel syndrome

doi: 10.1038/s41598-024-55258-4

Figure Lengend Snippet: Polyamines enhance mucosal defense factors in rats with massive intestinal resection. (A-B) Fecal and serum secretory IgA was measured using ELISA. n = 5–7/group. (C) IgA in ileum tissue was assessed using western blotting. n = 4–6/group. (D) Fecal mucin was measured using a fluorometric assay. n = 4–6/group. (E) Representative images of ileal villus showing immunostaining by Muc2 (original magnification, ×400; scale bars = 200 μm). Left graphs show the number of goblet cells per unit villous area and the size of goblet cell secretion granule. (F) The expression of Claudin-3 in the ileum tissue was measured by western blot analysis. Representative images of ileal villus showing immunostaining by Claudin-3 (original magnification, ×200; scale bars = 100 μm). (G) Serum DAO was measured by ELISA. n = 5–7/group. (H) Serum GLP-2 was measured by ELISA. n = 5–7/group. (I) Fecal short-chain fatty acid (SCFA) content was measured using high-performance liquid chromatography. n = 3–6/group. Data are presented as mean ± SD. Results of one-way ANOVA are represented as follows: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Nonspecific antibody binding was blocked using 15% goat serum for 30 min. For staining of Ki-67, Muc2, and Claudin-3, the slides were then incubated with anti-Ki67 antibody (ab16667; Abcam, Cambridge, UK), anti-Muc2 rabbit monoclonal antibody (M01212-1; Boster Biological Technology, Pleasanton, CA, USA), and Claudin-3 polyclonal antibody (#PA5-32353; Invitrogen, Waltham, MA, USA) at 4°C overnight, respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Immunostaining, Expressing, High Performance Liquid Chromatography

Journal: Molecular Medicine

Article Title: Evaluation of two laboratory model methods for diarrheal irritable bowel syndrome

doi: 10.1186/s10020-022-00599-x

Figure Lengend Snippet: The primers used in this experiment

Article Snippet: The blocking solution is gently shaken off and a proportion of primary antibodies ZO-1 (BOSTER PB9234, Wuhan, China) and MUC2 (BOSTER BM5029, Wuhan, China) in PBS is added dropwise to the sections, which are incubated overnight at 4 °C in a wet box.

Techniques: Sequencing

Journal: Molecular Medicine

Article Title: Evaluation of two laboratory model methods for diarrheal irritable bowel syndrome

doi: 10.1186/s10020-022-00599-x

Figure Lengend Snippet: Intensity of ZO-1 and MUC2 (green) in ileal and colonic tissues of rats in each group. Nuclei stained with DAPI (blue) (200×) ( A ), mRNA expression levels of ZO-1 ( B ), MUC2 ( C ), OCLN ( D ), CLDN4 ( E ) in each group of rats.* p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The blocking solution is gently shaken off and a proportion of primary antibodies ZO-1 (BOSTER PB9234, Wuhan, China) and MUC2 (BOSTER BM5029, Wuhan, China) in PBS is added dropwise to the sections, which are incubated overnight at 4 °C in a wet box.

Techniques: Staining, Expressing

Journal: Pathogens and disease

Article Title: Bacillus subtilis RZ001 improves intestinal integrity and alleviates colitis by inhibiting the Notch signalling pathway and activating ATOH-1.

doi: 10.1093/femspd/ftaa016

Figure Lengend Snippet: Fig. 2 Effects of the bacterial strains on MUC2 expression. (A) Effect of the

Article Snippet: The supernatant was collected and then MUC2 levels were measured by a mouse MUC2 ELISA kit (CSB-E15065m, Cusabio, Wuhan, China).

Techniques: Expressing